| Date | Product | Starting Plasmid | Thermocycler Variables: #cycles / annealing temp / extension time | Gel Picture | |
|---|---|---|---|---|---|
| 6/23 | Q2-BACE2-QX | pEXPR BACE2 | 30 / 60C / 30s | Gel Picture 6/23 | |
| Q1-eYFP-Q2 | pEXPR TRE:eYFP | ||||
| Q1-BACE2-Q2 | pEXPR BACE2 | ||||
| Q2-eYFP-QX | pEXPR TRE:eYFP | ||||
| 6/24 | Q2-BACE2-QX | pEXPR BACE2 | 35 / 56.2C / 30s | Gel Picture 6/24 | |
| Q1-eYFP-Q2 | pEXPR TRE:eYFP | ||||
| Q1-BACE2-Q2 | pEXPR BACE2 | ||||
| Q2-eYFP-QX | pEXPR TRE:eYFP | ||||
| 6/25 | Q1-BACE2-Q2 | pEXPR BACE2 | 35 / 50.2C / 30s
| Gel Picture 6/25 | |
| Q2-BACE2-QX | pEXPR BACE2 | ||||
| Q2-BACE2-QX | pEXPR BACE2 | ||||
| Q1-eYFP-Q2 | pEXPR TRE:eYFP | ||||
| Q2-eYFP-QX | pEXPR TRE:eYFP | ||||
| 6/26 | Q2-BACE2-QX (plus betaine) | pEXPR BACE2 | 30 / 55C / 1m
| Gel Picture 6/26 | |
Q2-BACE2-QX (plus DMSO) | pEXPR BACE2 | ||||
Q1-BACE2-Q2 (plus betaine) | pEXPR BACE2 | ||||
Q1-BACE2-Q2 (plus DMSO) | pEXPR BACE2 | ||||
| 6/27 | Q2-BACE1-QX | pEXPR BACE1 | Gel Picture 6/27 | ||
| Q2-eYFP-QX | pEXPR eYFP | ||||
| 6/30 | Q1-eYFP-Q2 | pEXPR eYFP | Gel Picture 6/30 | ||
| Q1-BACE1-Q2 | pEXPR BACE1 | ||||
| 7/9 | Q2-eYFP-QX (for BACE2) | pEXPR eYFP | 35 / 58C / 1m with both DMSO and Betaine | Gel Picture 7/9 | |
| Q1-eYFP-Q2 (for BACE2) | pEXPR eYFP | ||||
| Q2-eYFP-QX (for BACE1) | pEXPR eYFP | ||||
| Q2-BACE2-QX | pEXPR BACE2 | ||||
| Q1-BACE2-QX | pEXPR BACE2 | ||||
| Q1-BACE1-QX | pEXPR BACE1 | ||||
| 7/10 | (miRNA generator stuff) | 35 / 58C / 1m with both DMSO and Betaine | Gel Picture 7/10 | ||
| (20 PCR products in all) | |||||
| 7/1011 | (miRNA generator stuff) | 30 / 61C / 30s | (see lab notebook | Gel Picture 7/11 | |
| (12 PCR products in all) | |||||
| 7/1116 | (miRNA generator stuff) | 30 / 61C 63C / 30s | 11 | ||
| (12 PCR products in all) |
...