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Upon detection of beta-amyloid plaques (by receptor protein binding or by miRNA sensing) a transcription factor is cleaved from the receptor protein (LilrB2 or PirB) to then promote expression of a miRNA as well as BACE2. This miRNA, in turn, down-regulates BACE1.
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Experiments
Seeding 24-well Plate......Transfection......FACS
| Experiment | |
|---|---|
| Experiment | |
Demonstrate effectiveness of miRNA in down-regulating BACE1 | |
Demonstrate inducible upregulation of BACE2 | with GFP-YFP |
Purchases
| Item | LinkPurchased? | |
|---|---|---|
| BACE2 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=76140order had been requested | |
| BACE1 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=78236no | |
| Bace2 activity assay | https://www.anaspec.com/products/product.asp?id=57836 | order has been requested |
Options for Directly Assaying BACE2 Expression
| Bace1 activity assay | http | |
| Link | Cost | Comments |
|---|---|---|
| ://www.anaspec.com/products/product.asp?id= | 57836$435 | This is a kit to directly measure BACE2 expression |
| http://www.researchgate.net/post/Which_are_the_dye_suitable_for_visualization_of_neuron_with_beta-amyloid_protein_and_plaque10 | ||
| atomic force microscopy, Pittsburgh compound B |
Appendix
data sets