...
Upon detection of beta-amyloid plaques (by receptor protein binding or by miRNA sensing) a transcription factor is cleaved from the receptor protein (LilrB2 or PirB) to then promote expression of a miRNA as well as BACE2. This miRNA, in turn, down-regulates BACE1.
Experiments
Experiments
Seeding 24-well Plate......Transfection......FACS
| Experiment | |
|---|---|
| Experiment | |
Demonstrate effectiveness of miRNA in down-regulating BACE1 | |
Demonstrate inducible upregulation of BACE2 | with -YFP |
Purchases
| Item | Link | Purchased? |
|---|---|---|
| BACE2 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=76140 | ordered |
| BACE1 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=78236no | |
| Bace2 activity assay | https://www.anaspec.com/products/product.asp?id=57836 | Anaspec says a scaled-down kit is not available. We will contact them about possibly receiving the kit in-kind/at a discount. |
Options for Directly Assaying BACE2 Expression
| Bace1 activity assay | http | |
| Link | Cost | Comments |
|---|---|---|
| ://www.anaspec.com/products/product.asp?id= | 57836$435 | This is a kit to directly measure BACE2 expression. Call tech support. |
| http://www.researchgate.net/post/Which_are_the_dye_suitable_for_visualization_of_neuron_with_beta-amyloid_protein_and_plaque10 | ||
| atomic force microscopy, Pittsburgh compound B |
Appendix
data sets