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- Determine whether your sample is BL1 or BL2 (see below). Ask us if unsure!
- BL2 sign and extra preparation are required while imaging BL2 samples
- Don't pipette or add to your samples on the microscope stage!
- Use our local BL2 manipulation area for that
- Transport samples in airtight secondary containers with latching lids.
- MIT users must amend their BRR to include the microscopy core.
- You need ~30 min of additional training to image BL2 samples!
- You must bring your PPE and secondary container for BL2 training! We will postpone your training if you do not bring these items.
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After fixation, most human and mouse tissues and cells are no longer pathogenic and therefore Risk Group 1. Fixed samples may be observed under standard BL1 laboratory safety conditions. Live non-human specimens such as mouse cell lines that do NOT contain human pathogens are also BL1.
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BL2+ specimens such as cells with active or live lentivirus are not permitted in the KIMCF.
Elevated BL2 Operation Requirements
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Elevating a room to BL2
BL2 specimens such as live human-derived cells require the temporary raising of a room to BL2 status with proper signage and decontamination at the end of your session. See below.
Pay attention to BL1 and BL2 signs outside of each microscope room.
Research Registration Requirements
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MIT researchers (internal users) must have approval under their biological research registration (BRR) for use of the materials. BRR status will be verified and you must amend your BRR to include the KI-MCF (rooms 76-280 and 76-281) as a location in Section 2. Here is the current BRR amendment form [⬇️ download]. Please coordinate the amendment with the EHS rep for your home department and CC core staffdirector Jeffrey Kuhn (jrkuhn@mit.edu) in your email. We need to screen the form too.
We cannot provide BL2 services to external entities. All BL2 entities MUST have an existing BRR to amend.
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- All BL-2 level work, REQUIRES lab coat, gloves, and safety glassesgoggles.
- Gloves are provided by the core facility. Users must bring any other PPE they require.
- Some BL2-level work requires a face mask. Consult the exposure control plan in your BRR.
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