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 UntransfectedeBFP + Syk-TEVp + BCR (no Gal4VP16)eBFP + Syk-TEVp + BCR (no Gal4VP16) + UAS:mKateeBFP + BCR (CD79A-TCS-G4, CD79B) + UAS:mKateeBFP + BCR (CD79A, CD79B-TCS-G4) + UAS:mKateeBFP + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate
 

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Transfection Efficiency: ~20%

eBFP + BCR (CD79A-TCS-G4, CD79B) + UAS:mKateeBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate [0nM dox]eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate [1nM dox]eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate [10nM dox]eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate [100nM dox]eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate [1000nM dox]eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B) + UAS:mKate [2000nM dox]

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Cotransfection Efficiency: ~4%

eBFP + BCR (CD79A, CD79B-TCS-G4) + UAS:mKateeBFP + Syk-TEVp + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate [0nM dox]eBFP + Syk-TEVp + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate [1nM dox]eBFP + Syk-TEVp + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate [10nM dox]eBFP + Syk-TEVp + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate [100nM dox]eBFP + Syk-TEVp + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate [1000nM dox]eBFP + Syk-TEVp + BCR (CD79A, CD79B-TCS-G4) + UAS:mKate [2000nM dox]

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Cotransfection Efficiency: ~3.3%

eBFP + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKateeBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate [0nM dox]eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate [1nM dox]eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate [10nM dox]eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate 100nM dox]eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate [1000nM dox]eBFP + Syk-TEVp + BCR (CD79A-TCS-G4, CD79B-TCS-G4) + UAS:mKate [2000nM dox]

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Cotransfection efficiency: ~3.3%