Two delivery groups presenting on Tuesday!!!

Spinal Tap:

• Migratory?
• CSF Flow 
• Meninge? Layers?

Microneurosurgery 

• How hard/dangerous is it to inject something into the brain (purely the mechanical process, the cells being dangerous themselves is different)?
• are there concerns about even distribution (will it require multiple injections?  does getting to the inside of the brain require poking needles into it and does that cause problems?)

Viral Delivery 

• Research HSV1
  • What cells can we target
  • How much DNA can we pack in it
  • How does it integrate
  • Does it cause side effects
    • I assume it won't self replicate and cause a brain infection
    • how much do we need to get into the brain?
      • will this require multiple doses?
    • Cancer?

Exosomes

• Targeting

Microglia

• Ask a radiologist if two doses of 550 cGy is clinically meaningful and if there are differences between mice and humans when it comes to radiation and the brain
• papers
  • http://www.jci.org/articles/view/19205
    • Mice treated for genetic disease by using lentivirus to alter hematopoitic stem cells ex vivo which then fixed a brain disorder
  • http://www.nature.com/nm/journal/v7/n12/abs/nm1201-1356.html
    • microglia can be put into the brain, requires 2 doses of 550 cGy

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