Please move your personal culture collection to lab. stock when you graduate. Stock tubes, glycerols and mediums are supplied. Ask Yutaka.
BOX 01 (Ocean Microbes / Microbes require salinity)
| NAME | NOTE | SUGGESTED MEDIUM/CONDITION | ANTIBIOTICS | Made By | ||
|---|---|---|---|---|---|---|---|
A1 | Vibrio alginolyticus 12G01 |
| 2216 / RT~30 |
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A2 | Vibrio alginolyticus YM4 |
| 2216 / RT~30 |
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A3 | P. haloplanktis |
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A4 | P. haloplanktis |
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A5 | Vibrio cycrotrophicus ZF30 |
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A6 | Vibrio cycrotrophicus ZF99 |
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A7 | Vibrio cycrotrophicus ZF205 |
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A8 | Vibrio cycrotrophicus ZF28 |
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A9 | Vibrio cycrotrophicus FF160 |
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B1 | Vibrio cycrotrophicus 1F53 |
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B2 | Vibrio cycrotrophicus |
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B3 | Vibrio alginolyticus NMB102 | backward smooth swimming | Mixture |
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B4 | Vibrio alginolyticus NMB136 | forward smooth swimming | Mixture |
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B5 | Vibrio alginolyticus NMB102 | backward smooth swimming | Single Colony |
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B6 | Vibrio alginolyticus NMB136 | forward smooth swimming | Single Colony |
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B7 |
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B8 |
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B9 |
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C1 | Marinobacter aquaeolei VT8 (from ATCC) |
| frozen in 2216 media | n/a | steve | ||
C2 | Marinobacter aquaeolei VT8 (from ATCC) |
| frozen in Halomonas media | n/a | steve | ||
C3 |
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C4 |
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C5 |
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C6 |
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C7 |
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C8 |
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C9 |
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D1 |
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D2 |
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D3 |
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D4 |
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D5 |
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D6 |
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D7 |
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D8 |
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D9 |
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E1 |
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E2 |
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E3 |
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E4 |
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E5 |
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E6 |
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E7 |
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E8 |
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E9 |
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G1 |
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G2 |
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G3 |
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G4 |
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G5 |
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G6 |
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G7 |
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G8 |
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G9 |
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H1 |
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H2 |
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H3 |
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H4 |
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H5 |
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H6 |
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H7 |
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H8 |
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H9 |
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I1 |
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I2 |
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I3 |
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I4 |
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I5 |
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I6 |
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I7 |
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I8 |
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I9 |
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BOX 02 (Non-Ocean Microbes / Microbes doesn't require salinity)
| NAME | NOTE | SUGGESTED MEDIUM/CONDITION | ANTIBIOTICS | Made By |
|---|---|---|---|---|---|
A1 | E.Coli AW405 | from H. Berg | TB, LB |
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A2 | E.coli VS100 | smooth swimmer |
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A3 | E.coli VS100 | smooth swimmer |
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A4 | B. subtilis OI1085 |
| CAM Complete / ~37 |
| Marcos |
A5 | B. subtilis OI1085 |
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| Marcos |
A6 | B. subtilis OI4139 | smooth swimmer | CAM Complete + 1mM IPTG / ~37 |
| Marcos |
A7 | B. subtilis OI4139 | smooth swimmer |
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| Marcos |
A8 | B. subtilis OI4139 | smooth swimmer |
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| Marcos |
A9 |
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B1 |
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B2 |
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B3 |
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B4 |
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B5 |
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B6 |
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B7 |
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B8 |
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B9 |
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C1 | E. coli UU1615 | Parkinson lab, Tsr-only | tryptone |
| Noele |
C2 | E. coli UU1615 |
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| Noele |
C3 | E. coli UU1624 | Parkinson lab, Tar-only | tryptone |
| Noele |
C4 | E. coli UU1624 |
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| Noele |
C5 |
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C6 |
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C7 |
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C8 |
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C9 |
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D1 |
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D2 |
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D3 |
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D4 |
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D5 |
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D6 |
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D7 |
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D8 |
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D9 |
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E1 |
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E2 |
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E3 |
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E4 |
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E5 |
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E6 |
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E7 |
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E8 |
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E9 |
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G1 |
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G2 |
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G3 |
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G4 |
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G5 |
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G6 |
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G7 |
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G8 |
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G9 |
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H1 |
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H2 |
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H3 |
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H4 |
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H5 |
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H6 |
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H7 |
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H8 |
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H9 |
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I1 |
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I2 |
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I3 |
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I4 |
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I5 |
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I6 |
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I7 |
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I8 |
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I9 |
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BOX 09 (Non-Ocean Microbes / Microbes doesn't require salinity)
| NAME | NOTE | SUGGESTED MEDIUM/CONDITION | ANTIBIOTICS | MADE BY |
|---|---|---|---|---|---|
| A-B1 | PAO1ΔpslAB mCherry | Psl deficient strain - mCherry from Ribbeck's Lab (MIT) see Ref: DOI: 10.1371/journal.ppat.1003526 | 1% Tryptone Broth (TB) | Gentamicin, 50 μg mL−1 | Roberto (Jul 2014) |
| A-B2 | PAO1ΔpslAB | Psl deficient strain | 1% Tryptone Broth (TB) | Roberto (Jul 2014) | |
| A-B3 | PAO1-PBADpsl | Over-producing Psl requires Arabinose 2% final conc | 1% Tryptone Broth (TB) | Roberto (Jul 2014) | |
| A-B4 | PAO1ΔalgD | Alg deficient strain | 1% Tryptone Broth (TB) | Roberto (Jul 2014) | |
| A-B5 | PAO1ΔpelA | Pel deficient strain | 1% Tryptone Broth (TB) | Roberto (Jul 2014) | |
| A-B6 | PAO1 | wild type | 1% Tryptone Broth (TB) | Roberto (Jul 2014) | |
| A-B7 | PAO1 mCherry | wild type - mCherry | 1% Tryptone Broth (TB) | Gentamicin, 50 μg mL−1 | Roberto (Jul 2014) |
| A-B8 | PA14 | wild type | 1% Tryptone Broth (TB) | Roberto (Jul 2014) | |
| Pseudomonas aeruginosa PAO1 886 |
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| HJ |
| Pseudomonas aeruginosa PAO1 ΔlasR |
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| HJ |
| Pseudomonas aeruginosa PA14 2367 |
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| HJ |
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BOX 10 (Non-Ocean Microbes / Microbes doesn't require salinity)
| NAME | NOTES | SUGGESTED MEDIUM/CONDITION | ANTIBIOTICS | MADE BY |
|---|---|---|---|---|---|
1 | Pseudomonas aeruginosa ??? |
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| HJ |
2 | Pseudomonas aeruginosa ??? |
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| HJ |
3 | Pseudomonas aeruginosa PA14 ??? |
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| HJ |
4 | Pseudomonas aeruginosa ??? |
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| HJ |
5 | Pseudomonas aeruginosa PA14 - Flgk - YFP | Flagella mutant ??? |
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| HJ |
6 | Pseudomonas aeruginosa PA14 - Flgk - CFP | Flagella mutant ??? |
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| HJ |
7 | Pseudomonas aeruginosa ??? |
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| HJ |
8 | Pseudomonas aeruginosa ??? |
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| HJ |
9 | Pseudomonas aeruginosa ??? |
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| HJ |
10 | Pseudomonas aeruginosa ??? |
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| HJ |
| Pseudomonas aeruginosa Δpel | PA01 ???; matrix mutant ??? |
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| HJ |
| Pseudomonas aeruginosa PAO1 AlgD | alginate mutant ??? |
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| HJ |
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