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** PLEASE KEEP THE FILTER CUBES IN THE BASE STATE AFTER USE **

Filter sets from Chroma http://www.chroma.com

5X Fluorescence Filter Cubes: (EX: Excitation, DM: Dichromatic Mirror, BA: barrier filter emission)

c.f. To choose right filter combinations for fluorescent proteins -> [http://www.microscopyu.com/tutorials/java/fluorescence/fpfilterfinder/index.html]

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Microscope 1

 

1. RTDP : oxygen dye: EX:450/50nm, DM:485nm, BA: HQ550 (longpass, LP)

2. FITC -> EX:HQ480/40nm (HQ stands for high quality, i.e. high S/N ratio), DM:505nm, BA: HQ535/50nm Dyes:FITC, RSGFP, Bodipy, Fluo 3, Dio, GFP, Alexa488 [41001] 

3. Empty

4. Empty 

5. Empty  

6. Empty

Fluorescence Illuminator : X-cite 120 from Exfo

Objectives (Magnification / N.A.): 2X/0.08, 4x/0.13, 10x/0.30, 20x/0.45, 40x/0.60, 60x, 100x/1.40

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Microscope 2

 

1. Empty

2. FITC -> EX:HQ480/40nm (HQ stands for high quality, i.e. high S/N ratio), DM:505nm, BA: HQ535/50nm Dyes:FITC, RSGFP, Bodipy, Fluo 3, Dio, GFP, Alexa488 [41001] 

3. GREEN Chroma-> EX:546/10nm, DM:565nm, BA:590nm(longpass, LP) Dyes:TRITC, Rhodamine 237, RH414, RH421, RH795, LDS751, Proqidium Iodine, RFP [11002v2-GREEN]

4. Yellow GFP BP -> HQ500/20nm, DM:Q515nm (longpass, LP), BA: HQ535/30nm Dyes: YFP [41028]

5. UV -> EX:350/50nm (i.e. 325-375nm), DM:400nm (longpass, LP),  BA: 420nm (longpass, LP) Dyes:DAPI, Hoechst 34580, AMCA [11000v3-UV]

6. Cyan GFP -> D436/20nm, DM:455nm (longpass, LP), BA: D480/40nm Dyes: CFP [31044v2]

 

Note: The basic blue and green filter sets are designed primarily for viewing the fluorescence of a single fluorochrome. Both filter sets contain longpass emission filters which cannot exclude the fluorescence of a second fluorchrome which also may be excited. The basic green filter set is recommended for use with mercury lamps only.

Fluorescence Illuminator :

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Extra filter sets :  1) HPTS-> EX:425/40nm, DM:460nm (longpass, LP), BA: D540/50 Dyes: [31007]

                          2) BLUE Chroma-> EX: D470/40nm, DM:495nm (longpass, LP), BA: 515nm (longpass, LP) Dyes: Acridine Orange (both DNA & RNA), Auramine, EGFP, FITC, S65T, RSGFP[11001v2-BLUE]

                          3) Cy5 -> EX: 590/60 nm, DM: 660 (longpass, LP), BA: 638/75 Dyes: Cy5

 

 

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Scope 1 (at MIT until December 2015)

1.  FITC -> EX:HQ480/40nm (HQ stands for high quality, i.e. high S/N ratio), DM:505nm, BA: HQ535/50nm 

2. "Green" -> EX:546/10nm, DM:565nm, BA:590nm(longpass, LP)   (ring labeled: d546/10 x 61929)

3. empty

4. YFP ->  HQ500/20nm, DM:Q515nm (longpass, LP), BA: HQ535/30nm

5. "DAPI" -> EX:350/50nm (i.e. 325-375nm), DM:400nm (longpass, LP),  BA: 420nm (longpass, LP) [but labeled  550 LP] (ring labeled: d350/50 110295) 

6. "Cyan" -> D436/20nm, DM:455nm (longpass, LP), BA: D480/40nm

 

 

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