Our convention for storing and labeling your work is as follows:
Everyone's prefix is composed of their initials. For example, Ramez Danial's prefix is rd (lowercase).
Minipreps = [PREFIX]M[#]. For example, rdM1, rdM2, rdM3
Gel Preps = [PREFIX]G[#]. For example, rdG1, rdG2, rdG3
Restriction Digests = [PREFIX]R[#]. For example, rdR1, rd2, rdR3
Frozen Bacteria = [PREFIX]F[#]. For example, rdF1, rdF2, rdF3.
Frozen Yeast = [PREFIX]Y[#]. For example, rdY1, rdY2, rdY3.
See attached spreadsheet for how I like to keep track of my cell stocks.
I suggest maintaining multiple backups of your work to insure against catastrophies:
1) Store a frozen stock in the -80C. This can be done by adding 345 uL of 80% glycerol + 1.5 mL of healthy cell stock (preferably in late log phase but early stationary phase or overnight is usually OK).
2) Store a miniprepped version of the plasmid from the frozen stock in the -20C.