To Do
- Further BCR Research
- How is Lyn Phosphorylated?
- Is Syk Recruited Before Abeta binding
- Which Parts of the BCR proteins are extracellular/Intracellular
cd79's: N-signal peptide-extracellular domain-helical transmembrane-cytoplasmic-C - Switch TEV cleavage site and TEV Protease
- Why don't antibodies start with a Met (use self cleaving protein?)
- Turns out that the first few amino acids tell the cell that this will be put in the membrane and this signal tag is cleaved as part of post translation modification.
- Find this sequence for heavy and light chain
- Find Antibody Plasmid (multiple)
- Immunologist interview
- find all n and C termini on all proteins to know where to attach things
- syk
- near active site?
- lyn
- near active site?
- heavy
n is in variable region, c is in membrane - light chain
n is in variable region, c is in constant - cd79a
C terminus inside cell, n terminus outside and contains a membrane localization tag
C terminus near but separate from immunoreceptor tyrosine-based activation motif(ITAM) by ~30 amino acids - cd79b
same as cd79a
- syk
- Lab work
- Multiply plasmids
- Syk
- Lyn
- Verify? (sequence/gel)
- Multiply plasmids
- Experiments
- Planning
- add BCR steps
- Make Research Page
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