Overview
Upon detection of beta-amyloid plaques (by receptor protein binding or by miRNA sensing) a transcription factor is cleaved from the receptor protein (LilrB2 or PirB) to then promote expression of a miRNA as well as BACE2. This miRNA, in turn, down-regulates BACE1.
Experiments
| Experiment |
|---|
Determine which miRNA has greatest repressor potential for BACE1 |
Demonstrate effectiveness of miRNA in down-regulating BACE1 |
Demonstrate inducible upregulation of BACE2 with GFP
|
Purchases
| Item | Link | Purchased? |
|---|---|---|
| BACE2 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=76140 | ordered |
| BACE1 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=78236 | no |
| Bace2 activity assay | https://www.anaspec.com/products/product.asp?id=57836 | we will contact Anaspec about a smaller kit size |
Options for Directly Assaying BACE2 Expression
| Link | Cost | Comments |
|---|---|---|
| https://www.anaspec.com/products/product.asp?id=57836 | $435 | This is a kit to directly measure BACE2 expression. Call tech support. |
| http://www.researchgate.net/post/Which_are_the_dye_suitable_for_visualization_of_neuron_with_beta-amyloid_protein_and_plaque10 | ||
| atomic force microscopy, Pittsburgh compound B |
Appendix
data sets