Find which combination of TCS-Gal4VP16 proteins and what amount of Syk produces best Lyn on/off difference of reporter expression.
Try every combination of Gal4VP16 once with beta amyloid and once without.
Put Syk on dox inducible promoter (with mKate or something to measure Syk) and make a dox ladder for each test group to determine activity level.
| Standardized? information | ||||||
|---|---|---|---|---|---|---|
| Plasmids used | Protocols | Supplies | Control | Predicted results | Results | What this means |
CD79A CD79A-TCS-Gal4CP16 CD79B CD79B-TCS-Gal4CP16 Lyn dox inducible promoter Syk-15-TEVp dito mKate | ||||||
| Beta Amyloid? | Gal4VP16 on CD79A | Gal4V16 on CD79B |
|---|---|---|
0 | 0 | 0 |
| 0 | 0 | 1 |
| 0 | 1 | 0 |
| 0 | 1 | 1 |
| 1 | 0 | 0 |
| 1 | 1 | 0 |
| 1 | 0 | 1 |
| 1 | 1 | 1 |