NEED TO TES

Goal: Test the strength of different repressors

NameOnOffInducible
L7ae
  • Gateway Hef1a-2x Kturn-eYFP with pENTR_L1_L7ae_L2
  • Co transfect w/ constituative eBFP2
  • Flow Cytometry and analysis
  • Just Hef1a-2x Kturn-eYFP
  • Co transfect w/ constituative eBFP2
  • Flow Cytometry and analysis
  • rtTA & DOX?
TAL14
  • Gateway Hef1a-2x Kturn-eYFP with pENTR_L1_TAL14_L2
  • Co transfect w/ constituative eBFP2
  • Flow Cytometry and analysis
  • Just Hef1a-2x Kturn-eYFP
  • Co transfect w/ constituative eBFP2
  • Flow Cytometry and analysis
  • rtTA & DOX?

Someone upload Ron's morning meeting pictures, and explain them here

 

Notes:

2x-Kturn-eYFP from Nelson