How To Please Ron/Brain

• Keep updating the parts tree (include experiments)

miRNA Sensors (Theory)

• Figure out where the Golden Gates went
• Create protocol for siRNA transfection
• Meet with Jin at 3

miRNA Sensors (Lab Work)

• Transformations 
  • EF
  • GH
  • Mutant Hef1a-2xKturn-eGFP
  • Wild Type Hef1a-2xKturn-eGFP
• Pick colonies from L7ae and eBFP2
• Miniprep MAV1212
• Digest MAV1212
• Run gel for MAV1212
• Image gel for MAV1212

NOTES

Jin gave us ideas to optimize our high sensors and experiments