SAMPLE

Experiment Template:

  • 7/_: Seed cells
  • 7/_: Transfect Promoter:Gene (___ ng/uL) and Promoter:Gene (___ ng/uL) into HEK cells
  • 7/_ (_pm): Flow Cytometer

  • DNA Dilution: __uL Promoter:Gene; __uL Promoter:Gene
  • (Eg)x: add : (25uL Promoter:Gene-lipid complex)

 

Y1(Y+B)1//////////////////-ve control
  • dummy DNA
  • with Lipofectamine
Y2(Y+B)2//////////////////-ve control
  • dummy DNA
  • with Lipofectamine
B1(Y+B)3//////////////////-ve/-ve control
  • no DNA
  • no Lipofectamine
B2(Y+B)4//////////////////-ve/-ve control
  • no DNA
  • no Lipofectamine

Sample Experiment #2:

  • 7/3: Seed cells
  • 7/4: Transfect hEF1a:eYFP (472 ng/uL) and hEF1a:tagBFP (1176 ng/uL) into HEK cells
  • 7/6 (1pm): Flow Cytometer

 

Y1(Y+B)1//////////////////-ve control
  • dummy DNA
  • with Lipofectamine
Y2(Y+B)2//////////////////-ve control
  • dummy DNA
  • with Lipofectamine
B1(Y+B)3//////////////////-ve/-ve control
  • no DNA
  • no Lipofectamine
B2(Y+B)4//////////////////-ve/-ve control
  • no DNA
  • no Lipofectamine

 

Antibody Detection

 

Receptor Detection

 

miRNA Detection

 

Treatment