How To Please Ron/Brain

• Keep updating the parts tree (include experiments)

miRNA Sensors (Theory)

• Obtain pEXPR: hEF1a-GAL4VP16
• Fill out the Genewiz Order Form to sequence Low Sensor miR-144
• Prepare transfection well plate maps
• So where are the siRNA though...
• Why didn't the GH golden gate work on try #3?
• Change name of high sensors in tree

miRNA Sensors (Lab Work)

• Pick colonies
  • pEXPR: TAL14-mKate
• Midiprep
  • pEXPR: MAV1212-Hef11a-eBFP2
  • Mutant Hef1a-2xKturn-eGFP
  • Wild Type Hef1a-2xKturn-eGFP
  • Low Sensor miR-144
  • Hef1a-L7ae (LR)
• Miniprep
  • MAV1212-mRFP
  • L1_TALER14_L2
  • pTAL14-mKate-pEST
• LR
  • pEXPR: MAV1212-TRE-L7ae

NOTES

so...the siRNA are nowhere to be found

hEF1a-TAL14 (LR) has no colonies

TRE is nowhere to be found