TRANSFECTION PLANS

NameLocationConcentration
MAV1212-hEF1a-eBFP2Our -20Grown in midi culture
pEXPR:hEF1a-L7aeOur -20Grown in midi culture
pEXPR: hEF1a-2x Kturn-eGFPOur -20Grown in midi culture

 

EXPERIMENTS

  1. hEF1a eBFP2 & hEF1a 2x Kturn eYFP (positive control)
    1. Should be yellow and blue
  2. hEF1a eBFP2 & hEF1a 2x Kturn eYFP & hEF1a-L7ae (negative control)
    1. Should be blue (minimal yellow)

NameLocationConcentration
pEXPR: hEF1a-eBFP2Our -20Grown in midi culture
pEXPR: hEF1a-Gal4Vp16With Jin? Brian?Still looking
pEXPR: hEF1a-TAL14Out -20Grown in midi culture
pEXPR: 5xUAS/4xTAL14bs-eYFPWith Brian?UAS needs to be added somehow?

 

EXPERIMENTS:

  1. hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP
    1. negative control (minimal yellow)
  2. hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP & hEF1a-Gal4VP16
    1. positive control (hopefully linear BFP/YFP)
  3. hEF1a-eBFP2 & 5xUAS/4xTAL14bs-eYFP& hEF1a-Gal4VP16 & hEF1a-TAL14
    1. Should be blue (minimal yellow)