• PirB LR colonies picked and liquid cultures prepared 
• LilrB2 PCRs repeated under 2 different (higher) annealing temperatures 
• Remaining PirB PCR product ran and band with the correct size DNA to be extracted
• Gel with PCR products imaged 
  • Expected sizes:
    • LilrB2: 2,000
    • PirB: 2,500

  • HL

    LilrB2 (YFP)

    LilrB2 (TCS)

    PirB (YFP)

    PirB (TCS)