• PirB PCR bands extracted 
  • Concentrations very low 
    • around 5 ng/ul
• PirB PCRs run again 
• LilrB2 PCRs done at higher annealing temperatures 
• Cofilin plasmid colonies picked 
• PirB pEXPRs miniprepped 
• Restriction digest on PirB pEXPRs run