• Did PCR's of LilrB2
• Ran gel of all the PCR's
• Ladder, LilrB2 YFP 65, LilrB2 YFP 68, LilrB2 TCSG4 67, LilrB2 TCSG4 70, PirB YFP, PirB TCSG4, Cofilin YFP
• 

Did not want to put in imager (DNA degradation)

Looks like only Cofilin worked. Other bands are too big and also too light. Possibly PCR did not work.

Cofilin concentration ~10ng/uL

• Picked more pENTR_hEF1a: PirB colonies, in case the one is incorrect.