How To Please Ron/Brain

• Keep updating the parts tree (include experiments)

miRNA Sensors (Theory)

• Troubleshoot
  • Why didn't the oligos nanodrop??
  • Meet w/ Kyle about FlowJo
  • Look for the siRNA

miRNA Sensors (Lab Work)

• Golden Gate
  • Low Sensor miR-144-miR-181c
• Pick colonies
  
  • High Sensor miR-30d
  • High Sensor miR-146a
  • High Sensor let-7f
  • High Sensor miR-125b
  • Low Sensor miR-144
  • Low Sensor miR-181c
• Miniprep
  
  • pEXPR: MAV1212-TRE-L7ae (LR)
  • pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)
  • pEXPR: MAV1212-TRE-GAL4VP16 (LR)
• Restriction Digest & run gel
  • pEXPR: MAV1212-TRE-L7ae (LR)
  • pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)
  • pEXPR: MAV1212-TRE-GAL4VP16 (LR)
• Midiprep?
  • pEXPR: MAV1212-TRE-L7ae (LR)
  • pEXPR: MAV1212-hEF1a-GAL4VP16 (LR)
  • pEXPR: MAV1212-TRE-GAL4VP16 (LR)
• Gateway
  • pEXPR: MAV1212-hEF1a-TAL14
• Transform
  • pENTR: TAL14
  • pENTR: TAL21
  • pENTR: Something for yeast for Brian

NOTES

The gel didn't work very well, so we're re-doing it tomorrow

 CAG/CMV 2x Kturn Wild Type

 CAG/CMV 2x Kturn Wild Type