How To Please Ron/Brain

• Keep updating the parts tree (include experiments)
• Meet w/ Kyle
  • FlowJo
  • Bright Field Images

miRNA Sensors (Theory)

• Troubleshoot
  • What's going on with the double input low sensor
  • Is there a different GG thermal cycler program for Bbs1?
  • Run new transfection plate/protocol by Brian
• Move siRNA into our cryobox
• Grab sequencing primer from Jeremy's bench

miRNA Sensors (Lab Work)

• Pick colonies
  • pEXPR: MAV1212-hEF1a-TAL14 (Mini)
• Give plates to Brian
  • pENTR: TAL14p
  • pENTR: TAL21p
  • pENTR: Something for yeast for Brian
• Restriction Digest & run gel
  • pEXPR: MAV1212-TRE-L7ae (LR)
  • pEXPR: MAV1212-TRE-GAL4VP16 (LR)
• Transform
  
  • pEXPR: MAV1212-hEF1a-GAL4VP16
• Golden Gate 
  • All the sensors
• Split and Seed 4 plates

NOTES

We transfected way too much DNA --> new protocol with 1 ug total