How To Please Ron/Brain

• Keep updating the parts tree (include experiments)
• Meet w/ Kyle
  • FlowJo
  • Bright Field Images

miRNA Sensors (Theory)

• Troubleshoot
  • What's going on with the double input low sensor
  • Run new transfection plate/protocol by Brian
• Move siRNA into our cryobox
• Grab sequencing primer from Jeremy's bench

miRNA Sensors (Lab Work)

• Miniprep
  • pEXPR: MAV1212-hEF1a-TAL14
• Restriction Digest & run gel (4 samples)
  • pEXPR: MAV1212-TRE-L7ae (LR)
• Transform
  • pEXPR: MAV1212-TRE-GAL4VP16 (LR)
  • All the low sensors
• Transfect for L7ae Experiment 1.3
  
  

NOTES

We ran out of Lipofectamine and couldn't transfect