• Miniprep pEXPR (hEF1a: PirBTCS, hEF1a: TEVpCofilin)
• Verify hEF1a: PirBTCS
• Miniprep pENTR LilrB2TCS
  
• Verify pENTR LilrB2TCS
• Midiprep inoculate hEF1a: PirBTCS
• Pick more TREt:TEVpCofilin
• Plate more TREt:TEVpCofilin if possible.
  
  

Digest pEXPR_hEF1a: PirBTCSG4VP16 with BsrGI, buffer 2.1
Digest pENTR_LilrB2:TCSG4VP16 with XhoI +XbaI, buffer CS

Order: hEF1a PT 1, 2, 3, 4, LT16, 17, 18, 19

hEF1a: PT 1, 3 and 4 might have worked, given that I can't even see the 800bp of the ladder either. Needs further testing.
LT 16 might have worked: there is a very faint band just below the 1500 place. Should test further, but should also keep searching.

To Do:

Short

• Take out TC plate and liquid cultures
• Take out LT liquid cultures
• Miniprep ^

Long

• Find good TREt:TC <-- super important. Needs to get done by end of this week.
  
• Find good LT
• Do hEF1a:LT LR
• PCR C term YFP
•