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14-8-7

pENTR-L1-TALER14-L2 Digest AseI 3.1

MIT iGEM 2014 > JX August > pENTR-TAL14.png

14-8-8

LR Gateway

MAV1212-RFP
hEF1a
TAL14

MAV1212-RFP
hEF1a
TAL21

14-8-11

Transformation

MAV1212-hEF1a-TALER14

MAV1212-hEF1a-TALER21

14-8-15

MIT iGEM 2014 > JX August > 8-15.png

I dont understand i dont know i dont

14-8-18

LR
MAV1212-mRFP pENTR-L4-hEF1a-R1 pENTR-L1-TALER14-L2

14-8-19

Transformation
MAV1212-hEF1a-TALER14

14-8-20

Propagation

LR

MAV1212-hEF1a-TALER14

MAV1212-mRFP

pENTR-L4-hEF1a-R1

pENTR-L1-TALER14-L2

14-8-21

Digest	Transformation
MAV1212-hEF1a-TALER14:	MAV1212-hEF1a-TALER14

DNA	Con (ng/µL)	Enzyme	Buffer	Vol DNA	Mass DNA	Vol Enzyme	Vol Buffer	Vol Water	Vol Total
Ladder
TAL14 Insert 1	282.5	PstI	3.1	3.54	1000	1	2	13.46	20
TAL14 Insert 2	316.9	PstI	3.1	3.16	1000	1	2	13.84	20
TAL14 Control	316.9			3.16	1000			16.84	20

MIT iGEM 2014 > JX August > 8-21.png