What experiments are we planning to do with the neurons? 

• beta amyloid assay with app/appswe transfection about that..
  • we decided against thioflavin t because it binds to beta amyloid fibrils and we don't know whether or not it will bind oligomers
  • we think beta amyloid antibodies are probably our best option 
  • doing an ELISA assay is probably another option 
• action potentials with and without beta amyloid production
  • how long will it take 
• miRNA testing? 
• capstone/prelims in neurons? 

What neuron options do we have?

• brainbits neurons
• french sebastien neurons have channel rhodopsin so can fire and turn pretty colors
  • (but we have to differentiate those)
  • can we differentiate them into not motor neurons (more representative of alzheimer's disease)
  • we should talk to sebastien about how he manipulates his neurons/ if he's willing to give us some already differentiated cells
• sebastien palacio's neurons - "talk to eldi" sebastien
  • not rhodopsin-y --> not cool  
  • not motor neurons - more accurate representation of alzheimer's 
  • this sebastien is more likely to help us work with the neurons/ address any problems that we face

What do we need to know about the neurons? 

• how are they transfected?
• how long do they last?
• how long do they take to differentiate?