Possible Organisms

• Caldicellulosiruptor saccharolyticus
  • inputs:
  • outputs:
• E. Coli
• Geobacter
• rhodobacter
  • This paper describes hydrogen gas production by Rhodobacter sphaeroides O.U.001 using a column photobioreactor in batch and continuous operation (http://link.springer.com/chapter/10.1007%2F978-0-585-35132-2_18)
  • inputs: light, L-malic acid, and sodium glutamate
  • outputs: 
• enterobacter

More synbio approaches

• Getting another organism to take away the byproducts that hinder hydrogen production
  • combining dark fermentation and photo fermentation - Combined fermentation
  • getting an organism to consume the acetate
• containment - making the bacteria dependent on each other to survive
  • syntrophic exchange (http://www.pnas.org/content/111/20/E2149.short?rss=1)
  • down-regulate the pathways except for the hydrogen production pathway