hEF1a:CD79A-XnTCSXc-Gal4VP16
| 3TCS3 | 9TCS3 | 15TCS3 | ||
| 6TCS6 | 9TCS6 | 12TCS6 | ||
| no colonies | ||||
| 3TCS9 | 6TCS9 | 9TCS9 | 12TCS9 | 15TCS9 |
| 6TCS12 | 9TCS12 | 12TCS12 | ||
| 3TCS15 | 9TCS15 | 15TCS15 | ||
| All linkers | 9TCS6 |
|---|---|
annealed linker oligos golden gate 06/17 | |
| transformed 06/18 | |
| pick colonies 06/19 | retransformed 06/19 |