06/17

• annealed linker oligos
• golden gate into RFP construct 

06/18

• transformed golden gate product

06/19

• all linkers grew colonies except 9TCS6 
• picked colonies for all except 9TCS6
• retransformed 9TCS6

06/23 

• digest and gel verification 
• 3TCS3, 3TCS9, 9TCSX show correct band pattern

06/24

• sent 9TCSX for sequencing