06/17

  • annealed linker oligos
  • golden gate into RFP construct 

06/18

  • transformed golden gate product

06/19

  • all linkers grew colonies except 9TCS6 
  • picked colonies for all except 9TCS6
  • retransformed 9TCS6

06/23 

  • digest and gel verification 
  • 3TCS3, 3TCS9, 9TCSX show correct band pattern

06/24

  • sent 9TCSX for sequencing 

hEF1a:CD79A-XnTCSXc-Gal4VP16

3TCS3 9TCS3 

15TCS3

midi innoculated midi verified midi innoculated
 6TCS69TCS612TCS6 
 midi verifiedmidi verifiedmidi verified 
3TCS9

6TCS9

9TCS9

12TCS9

15TCS9

midi verifiedto be miniedmidi innoculatedto be miniedsequencing
 6TCS12

9TCS12

12TCS12

 
 midi verifiedto be miniedto be minied 
3TCS15 

9TCS15

 

15TCS15

sequencing to be minied midi innoculated