3/7/14

Made two LR reactions as practice. 

First aliquot, 4 uL total:

• 1 uL of 5 fmol tag-BFP
• 1 uL of 5 fmol Hef1a promoter
• 1 uL of 10 fmol Destination vector
• 1 uL of LR enzyme

Second aliquot, 4 uL total: 

• 1 uL of 5 fmol tag-BFP
• 1 uL of 5 fmol TREt promoter
• 1 uL of 10 fmol Destination vector
• 1 uL of LR enzyme

Stayed on the counter at room temperature overnight