14-Mar-7









LR Reaction:






 Label 
 


 Gene 
 


 (fmol) 
 


 Promoter 
 


 (fmol) 
 


 Destinatioin 
 


 (fmol) 
 


 LR Clonase(uL) 


 Total Volume 
 






 JX 1 
 


 mKate 
 


 5 
 


 Het1a-LacO 
 


 5 
 


 1-2 
 


 10 
 


 1 
 


 4 
 






 JX 2 
 


 rtTA 
 


 5 
 


 Het1a 
 


 5 
 


 1-2 
 


 10 
 


 1 
 


 4 
 








14-Mar-14






Gibson Reaction Mix



Sample Y166







 


 DNA 


 I-Sce-1 


 Buffer 


 Water 


 Total 






 Mass (μg) 


 1 


 


 


 


 






 Concentration   (ng/μL) 


 316 


 


 


 


 






 Volume (μL) 


 3.16 


 1 


 2 


 13.84 


 20 






 Order 


 2 


 4 


 3 


 1 


 







Created Gibson reactio mix. Reactants entered in volume and order noted in table.