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14-Mar-7

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LR Reaction:
|| Label \\ || Gene \\ || (fmol) \\ || Promoter \\ || (fmol) \\ || Destinatioin \\ || (fmol) \\ || LR Clonase(uL) || Total Volume \\ ||
| JX 1 \\ | mKate \\ | 5 \\ | Het1a-LacO \\ | 5 \\ | 1-2 \\ | 10 \\ | 1 \\ | 4 \\ |
| JX 2 \\ | rtTA \\ | 5 \\ | Het1a \\ | 5 \\ | 1-2 \\ | 10 \\ | 1 \\ | 4 \\ |
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14-Mar-14

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Gibson Reaction Mix

Sample Y166
| | DNA | I-Sce-1 | Buffer | Water | Total |
| Mass (μg) | 1 | | | | |
| Concentration   (ng/μL) | 316 | | | | |
| Volume (μL) | 3.16 | 1 | 2 | 13.84 | 20 |
| Order | 2 | 4 | 3 | 1 | |
Created Gibson reactio mix. Reactants entered in volume and order noted in table.

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14-Mar-16

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Gibson Reaction Mix for All Samples

| Mass (μg) | Enzyme (μL) | Buffer (μL) | Total (μL) | |
| 1 | 1 | 2 | 20 | |
| | | | | |
| | Con (ng/μL) | Vol DNA (μL) | Vol H2O (μL) | |
| 1.1 | 151 | 6.62 | 10.38 | CMU-Nde1 |
| 1.2 | 267 | 3.75 | 13.25 | |
| 1.3 | 141 | 7.09 | 9.91 | |
| 2.1 | 652 | 1.53 | 15.47 | TRE-t-Sta1 |
| 2.2 | 263 | 3.80 | 13.20 | |
| 2.3 | 212 | 4.72 | 12.28 | |
| 4.1 | 149 | 6.71 | 10.29 | TRE-t-Sta1 |
| 4.2 | 415 | 2.41 | 14.59 | |
| 4.3 | 583 | 1.72 | 15.28 | |
| 6.1 | 353 | 2.83 | 14.17 | TRE-t-Sta1 |
| 6.2 | 593 | 1.69 | 15.31 | |
| 6.3 | 361 | 2.77 | 14.23 | |
| 7.1 | 448 | 2.23 | 14.77 | Het1a-Xhol |
| 7.2 | 237 | 4.22 | 12.78 | |
| 7.3 | 186 | 5.38 | 11.62 | |
| 8.1 | 246 | 4.07 | 12.93 | Het1a-Lac-BamH1 |
| 8.2 | 182 | 5.49 | 11.51 | |
| 8.3 | 871 | 1.15 | 15.85 | |
| 9.1 | 410 | 2.44 | 14.56 | TRE-t-Sta1 |
| 9.2 | 214 | 4.67 | 12.33 | |
| 9.3 | 185 | 5.41 | 11.59 | |
| 10.1 | 285 | 3.51 | 13.49 | CMU-Nde1 |
| 10.2 | 547 | 1.83 | 15.17 | |
| 10.3 | 388 | 2.58 | 14.42 | |
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Gel Figure for Previous Samples:


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