To Do

• Further BCR Research
  •  How is Lyn Phosphorylated?
  • Is Syk Recruited Before Abeta binding
  • Which Parts of the BCR proteins are extracellular/Intracellular
  • Switch TEV cleavage site and TEV Protease
  • Why don't antibodies start with a Met (use self cleaving protein?)
    • Turns out that the first few amino acids tell the cell that this will be put in the membrane and this signal tag is cleaved as part of post translation modification.
    • Find this sequence for heavy and light chain
  • Find Antibody Plasmid (multiple)
  • Immunologist interview
  • find all n and C termini on all proteins to know where to attach things
• Lab work
  • Multiply plasmids
    • Syk
    • Lyn
  • Verify? (sequence/gel)
• Experiments
• Planning
  • add BCR steps
• Make Research Page

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