added:

1uL of TRE-t (1) @ 5fM

1uL of EYFP @ 5fM

1uL of pDEST 12 @ 10fM

0.5uL of clonase

mixed by pipetting, a few air bubbles in aliquot.

left over night at room temperature

Purpose: practice

added:

1uL of Hef1a (1) @ 5fM

1uL of EBFP @ 5fM

1uL of pDEST 12 @ 10fM

0.5uL of clonase

mixed by pipetting, one air bubble in aliquot.

left over night at room temperature

Purpose: practice