Subgroup Specific

Treatment: 

• N and C terminus fusion

Antibody:

• Low DNA concentration (probably because incubator was not on all night) 
• Sequence DNA
• Meeting with immunologist 
• Pick another colony - grow it and miniprep it tomorrow 
• Fusion design - design under assumption that kozak sequence is there, but sequence it to make sure

Receptor: 

• • Liquid culture of picked colonies didn't grow 
  • when we pick new colonies, put it in incubator on 2nd floor
  • negative control didn't turn blue 
    • plates are not the right kind
    • mutation in lacZ
    • likely that reaction happened successfully 
      
      • to check: miniprep dna from colonies, digest it and run a gel to figure out whether or not the gblocks are there 
      • sequence dna and look for mutations (frameshifts, stop codon, L sites)