Antibodies 

• Did a lot of PCR yesterday 
  • Manufacturer of Phusion mix used in PCR will detail what variables to use in PCR based on the primers and templates you are using
• Purify PCR product so that you can measure concentrations and use correct amounts for Golden Gate

Receptor

• Check that centrifuge is actually running 
• Transfect today 
• Cytometer  on thursday 
  

miRNA

• Meeting with Jake Beal 
  • depending on type of sensor you may be able to have 12 miRNA 
  • co-transfecting may be better than putting everything on the same plasmid

NEGEM NOTES

• Science is hard 
• Our project is cool/really hard 
• HP is really important 
  • ethics and legal stuff 
  • interview professionals, laymen, patients and families 
• Potential delivery methods and what would treatment look like 
• what is the current state of the art...
  • Diagnosis? 
  • Treatment? 
• BioBricks and the Parts Registry 
  • Mammalian Parts 
  • Overview 
  • What are our responsibilities?