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June 19
To do:
- High school presentations
- Re-culture Syk and Lyn
- Update our section of the parts list
- Design Lyn-TEVp fusion
Notes:
- Syk and Lyn concentrations found to be only ~15 ng/uL, so need to grow new cultures and re-miniprep
- Primers iGEM2014-EE-[003,006] found to be missing spacer before BsaI site; corrected primers re-ordered
June 20
To do:
- Miniprep Syk/Lyn and nanodrop
- Sequence Syk/Lyn
- Discuss antibody orders with Brian
- NEGEM
June 22
To do:
- All the PCRs!
- CD79A + stop codon
- CD79A + TCS
- mKate (for fusion proteins)
- Sequence Syk/Lyn
June 23
To do:
- Image gel from June 22
- Golden Gate PCRs from June 22
- More PCRs with new primers
June 25
To do:
- Redo Syk-eYFP PCR
- Image gel for yesterday's PCRs
- Purify and gel Syk-eYFP PCR
- Run remaining PCRs
- Purify and gel remaining PCRs
- Golden Gates
- Put gel images on parts pages
- Update wiki
Notes:
- All primers have now arrived.
- CD79B PCRs failed again; we might need to design new primers.