Results
| tube name | Hyper Ladder 1kb | pZDonor 3-GTW-4 | pEXPR hef1a:CD79B-TCS-Gal4VP16 1 | pEXPR hef1a:CD79B-TCS-Gal4VP16 1 | pEXPR hef1a:CD79B-TCS-Gal4VP16 2 | pEXPR hef1a:CD79B-TCS-Gal4VP16 3 |
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 |
| DNA mass desired (ng) | 500 | 500 | 500 | 500 | 500 | 500 |
| total volume(ul) | 20 | 20 | 20 | 20 | 20 | 20 |
| DNA concentration (ng/ul) | 132 | 132 | 142 | 110 | ||
| Enzyme Name | ApaI | undigested | ApaI | ApaI | ApaI | |
| Buffer Name | ||||||
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 |
| DNA volume | #DIV/0! | #DIV/0! | 3.79 | 3.79 | 3.52 | 4.55 |
| buffer volume | 2 | 2 | 2 | 2 | 2 | 2 |
| enzyme volume | 1 | 1 | 1 | 2 | 3 | 4 |
| water volume | #DIV/0! | #DIV/0! | 13.2 | 12.2 | 11.5 | 9.5 |
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 |
| loading dye | 4 | 4 | 4 | 5 | 6 | 7 |
| result | ||||||
| notes | couldn't find |
Virtual gels from Geneious
