Filter sets from Chroma http://www.chroma.com
5X Fluorescence Filter Cubes: (EX: Excitation, DM: Dichromatic Mirror, BA: barrier filter emission)
1. RTDP
2. FITC -> EX:HQ480/40nm (HQ stands for high quality, i.e. high S/N ratio), DM:505nm, BA: HQ535/50nm Dyes:FITC, RSGFP, Bodipy, Fluo 3, Dio, GFP, Alexa488 [41001]
3. Yellow GFP BP -> HQ500/20nm, DM:Q515nm (longpass, LP), BA: HQ535/30nm Dyes: YFP [41028]
4. GREEN-PROCL-> EX:546/10nm, DM:565nm, BA:590nm(longpass, LP) Dyes:GFP [11002v2-GREEN]
5. Cyan GFP -> D436/20nm, DM:455nm (longpass, LP), BA: D480/40nm Dyes: CFP [31044v2]
6. Currently Empty
Extra two filter sets : 1) HPTS-> EX:425/40nm, DM:460nm (longpass, LP), BA: D540/50 Dyes: [31007]
2) UV -> EX:350/50nm (i.e. 325-375nm), DM:400nm (longpass, LP), BA: 420nm (longpass, LP) Dyes:DAPI, Hoechst, AMCA [11000v3-UV]
3) BLUE-PROCL-> EX: D470/40nm, DM:495nm (longpass, LP), BA: 515nm (longpass, LP) Dyes: [11001v2-BLUE]
c.f. To choose right filter combinations for fluorescent proteins -> http://www.microscopyu.com/tutorials/java/fluorescence/fpfilterfinder/index.html
Fluorescence Illuminator : X-cite 120 from Exfo
Objectives (Magnification / N.A.): 2X/0.08, 4x/0.13, 10x/0.30, 20x/0.45, 40x/0.60, 60x, 100x/1.40
CCD Camera :PCO 1600