Project Planning

Meeting notes 6/16

To Do

• Further BCR Research
  •  How is Lyn Phosphorylated?
  • Is Syk Recruited Before Abeta binding
    no, it's recruited from the cytoplasm upon receptor activation
    
  • Which Parts of the BCR proteins are extracellular/Intracellular
    cd79's: N-signal peptide-extracellular domain-helical transmembrane-cytoplasmic-C
  • Switch TEV cleavage site and TEV Protease
    doesn't work because of nuclear localization
  • Why don't antibodies start with a Met (use self cleaving protein?)
    • Turns out that the first few amino acids tell the cell that this will be put in the membrane and this signal tag is cleaved as part of post translation modification.
    • Find this sequence for heavy and light chain
      • verify function of our sequence
        • find someone who is expressing membrane bound antibodies
        • check immunotherapy, perhaps cancer?
  • Find Antibody Plasmid (multiple)
  • Immunologist interview
  • find all n and C termini on all proteins to know where to attach things
    • syk
      attaching to C terminus works (even though it seems relatively close to active site); N terminus is further from active site
    • lyn
      • near active site?
    • heavy
      n is in variable region, c is in membrane
    • light chain
      n is in variable region, c is in constant
    • cd79a
      C terminus inside cell, n terminus outside and contains a membrane localization tag
      C terminus near but separate from immunoreceptor tyrosine-based activation motif(ITAM) by ~30 amino acids
    • cd79b
      same as cd79a
• Lab work
  • Multiply plasmids
    • Syk
    • Lyn
  • Verify? (sequence/gel)
• Experiments
• Planning
  • add BCR steps
• WIki stuff
  
  • Make Research Page
  • use cloaks to make this list collapsible

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