| tube name | Hyper Ladder 1kb | pZDonor 1-2 | pEXPR Lyn-TEVp 2 | pEXPR Lyn-TEVp 1 | pEXPR Lyn-TEVp 2 | ggDonor | Syk-eyfp 1 | Syk-eyfp 1 | Syk-eyfp 2 | Syk-eyfp 3 | Syk-eyfp 4 | Syk-eyfp 5 |
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| DNA mass desired (ng) | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 | 500 |
| total volume(ul) | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 | 20 |
| DNA concentration (ng/ul) | 377.9 | 286.9 | 22.3 | 286.9 | 105.2 | 509.9 | 509.9 | 410 | 528.9 | 247.4 | 512.1 | |
| Enzyme Name | BsaI | undigested | BsaI | BsaI | NsiI | undigested | NsiI | NsiI | NsiI | NsiI | NsiI | |
| Buffer Name | ||||||||||||
| gel/lane | 1 | 2 | 3 | 4 | 3 | 6 | 7 | 4 | 9 | 10 | 5 | 12 |
| DNA volume | #DIV/0! | 1.32 | 1.74 | 22.42 | 1.74 | 4.75 | 0.98 | 0.98 | 1.22 | 0.95 | 2.02 | 0.98 |
| buffer volume | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 2 |
| enzyme volume | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| water volume | #DIV/0! | 15.7 | 15.3 | -5.4 | 15.3 | 12.2 | 16.0 | 16.0 | 15.8 | 16.1 | 15.0 | 16.0 |
| gel/lane | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| loading dye | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 | 4 |
| results | ||||||||||||
| notes |
Virtual gels from Geneious
| pEXPR Lyn-TEVp | pZDonor 1-GTW-2 | GGDonor | Syk-eyfp |
|---|---|---|---|
 |  |  |  |