Overview
Upon detection of beta-amyloid plaques (by receptor protein binding or by miRNA sensing) a transcription factor is cleaved from the receptor protein (LilrB2 or PirB) to then promote expression of a miRNA as well as BACE2. This miRNA, in turn, down-regulates BACE1.
Experiments
| Experiment |
|---|
Determine which miRNA has greatest repressor potential for BACE1 |
Demonstrate effectiveness of miRNA in down-regulating BACE1 |
Demonstrate inducible upregulation of BACE2 with YFP |
Demonstrate ability to down-regulate EP2 expression |
Purchases
| Item | Link | Purchased? |
|---|---|---|
| BACE2 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=76140 | received |
| BACE1 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=78236 | received |
| Bace2 activity assay | https://www.anaspec.com/products/product.asp?id=57836 | We have contacted them about possibly receiving the kit in-kind/at a discount. |
| Bace1 activity assay | http://www.anaspec.com/products/product.asp?id=57888 | We have contacted them about possibly receiving the kit in-kind/at a discount. |
| PTGER2 | http://dnasu.org/DNASU/GetCloneDetail.do?cloneid=305296 |
Appendix
data sets