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In this experiment, we will determine the affinity of beta-amyloid for the variable regions of our mIgM in the B-cell receptor.
We plan to determine whether beta-amyloid binds to our BCR. We would also like to establish a Kd value if possible.
Well 1
HEK293
Well 2
Dummy DNA
Well 3
hEF1a: GmabL
hEF1a: GmabH
hEF1a: CD79A
hEF1a: CD79B
hEF1a: mKate
Well 4
anti IgM
Well 5
Well 6
Well 7
Well 8
Well 9
1000 nm abeta
Well 10
EMPTY
Well 11
Well 12
Well 13
Well 14
Well 15
Well 16
Well 17
Well 18
Well 19
Well 20
Well 21
Well 22
Well 23
Well 24
http://www.renyi.hu/~stipsicz/skin/Flow_cytometry_book/Ch_17.pdf p.16-19 - protocol for yeast using c-myc as transfection marker