You are viewing an old version of this page. View the current version.
Compare with Current View Page History
« Previous Version 8 Next »
In this experiment, we will determine the affinity of beta-amyloid for the variable regions of our mIgM in the B-cell receptor.
We plan to determine whether beta-amyloid binds to our BCR. We would also like to establish a Kd value if possible.
Well 1
HEK293
Well 2
Dummy DNA
Well 3
hEF1a: eYFP
Well 4
hEF1a: GmabL
hEF1a: GmabH
hEF1a: CD79A
hEF1a: CD79B
Well 5
1000 nM abeta
Well 6
Anti IgM
Well 7
Well 8
Well 13
Well 14
Well 15
Well 16
Well 17
Well 18
Well 19
Well 20
Well 21
Well 22
Well 23
Well 24
http://www.renyi.hu/~stipsicz/skin/Flow_cytometry_book/Ch_17.pdf p.16-19 - protocol for yeast using c-myc as transfection marker