Active TANGO Test

This test will vary several circuit parameters to find the ideal sensor and characterize total sensor behavior.

This will determine what amounts of Syk-TEVp for each placement of TCS-Gal4VP16 produce the best on/off ratio.

BCR (CD79A+B (with and without TCS-Gal4VP16), IgM heavy, IgM light), Lyn, Syk-TEVp, tre-mKate(dox reporter circuit), UAS-eYFP(reporter circuit), hef1a:eBFP(transfection marker), (whatever is needed to make dox work).  We will be testing all combinations of CD79(A/B)-(stop/TCS-Gal4Vp16).

Dox, beta amyloid (if beta amyloid fails, anti IgM can be used to force the receptor into an active state)

All combinations of plasmids being tested will be prepared and put into dox ladders

First row is control for basal reporter expression.

We should identify what level of Syk-TEVp produces the best on/off ratio. Our data will produce a scatter plot of eYFP (reporter) as a function of mKate (driven by the same promoter as syk).