Test using the whole detection circuit and Beta Amyloid to measure circuits response to input
Purpose
This will determine the ideal concentration of Syk-TEVp for the best signal/noise ratio as well as characterize our circuit for use in other circuits.
Parts Needed
BCR (CD79A+B, IgM heavy, IgM light), Lyn, Syk-TEVp, (reporter circuit), (whatever is needed to make dox work). We will be testing all combinations of CD79(A/B)-(stop/TCS-Gal4Vp16).
Dox, Beta Amyloid
Setup
All combinations of plasmids being tested will be prepared and put into dox ladders
Expected Results
The Expression of the reporter should be much higher in Beta Amyloid than in the previous Beta amyloid- test.