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3/7/14

Made two LR reactions as practice. 

First aliquot, 4 uL total:

  • 1 uL of 5 fmol/uL tag-BFP
  • 1 uL of 5 fmol/uL Hef1a promoter
  • 1 uL of 10 fmol/uL Destination vector
  • 1 uL of LR enzyme

Second aliquot, 4 uL total: 

  • 1 uL of 5 fmol/uL tag-BFP
  • 1 uL of 5 fmol/uL TREt promoter
  • 1 uL of 10 fmol/uL Destination vector
  • 1 uL of LR enzyme

Stayed on the counter at room temperature overnight

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