Parts:

Ig-alpha (CD79A), Ig-beta (CD79B), IgM (one of these will be fused with TEV sequence/transcription factor)

Lyn (fused with TEV protease)

 

Further Research:

  • Lyn expression in neurons
  • Disulfide bonds between Ig-alpha and Ig-beta
  • Expressing parts: how big are they, can we order gBlocks?
  • What antibody do we use? -> is it patented?

 

Preliminary Experiments:

  • co-immunoprecipitation to see if Ig-alpha/Ig-beta complex is forming
    • use biotinylated anti-Ig-alpha and anti-Ig-beta antibodies
    • add these antibodies to "cell soup"
    • run over streptavidin column
    • in gel: if Ig-alpha comes off with Ig-beta, or Ig-beta came off with Ig-alpha -> suspect that they were bound
  • Lyn recruitment
    • fluorescence to check localization?
  • biotinylated beta-amyloid to see if it sticks to cells

 

DNA Parts to Order:

Antibody against beta-amyloid: Gantenerumab

  • light chain (220aa) -> specific to beta-amyloid
  • heavy chain (~500aa) -> modified so that it is IgM rather than IgG
  • Ig-alpha
  • Ig-beta
  • Lyn
  • beta-amyloid (to test)

 

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