Fluorescent localization

Dox ladder

Activated/inactivated BCR (plus)/(minus) anti-IgM (photobleached?) or beta-amyloid

36 separate wells

 Lyn-mKateSyk-eYFP
(minus) Lyn(plus) Lyn
Dox0 nM(minus)(minus)(minus)
(plus)(plus)(plus)
1 nM(minus)(minus)(minus)
(plus)(plus)(plus)
10 nM(minus)(minus)(minus)
(plus)(plus)(plus)
100 nM(minus)(minus)(minus)
(plus)(plus)(plus)
1000 nM(minus)(minus)(minus)
(plus)(plus)(plus)
2000 nM(minus)(minus)(minus)
(plus)(plus)(plus)

 

 

Capstone

 

  Lyn-TEVpSyk-TEVp
CD79BCD79A(minus) Lyn(plus) Lyn
(minus) TCS-G4(minus) TCS-G4

Lyn-TEVp

CD79A

CD79B

Syk-TEVp

CD79A

CD79B

Syk-TEVp

Lyn

CD79A

CD79B

(plus) TCS-G4

Lyn-TEVp

CD79A-TCS-G4

CD79B

Syk-TEVp

CD79A-TCS-G4

CD79B

Syk-TEVp

Lyn

CD79A-TCS-G4

CD79B

(plus) TCS-G4(minus) TCS-G4

Lyn-TEVp

CD79A

CD79B-TCS-G4

Syk-TEVp

CD79A

CD79B-TCS-G4

Syk-TEVp

Lyn

CD79A

CD79B-TCS-G4

(plus) TCS-G4

Lyn-TEVp

CD79A-TCS-G4

CD79B-TCS-G4

Syk-TEVp

CD79A-TCS-G4

CD79B-TCS-G4

Syk-TEVp

Lyn

CD79A

CD79B-TCS-G4

 

 

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